Choline intake and genetic polymorphisms influence choline metabolite concentrations in human breast milk and plasma.

TitleCholine intake and genetic polymorphisms influence choline metabolite concentrations in human breast milk and plasma.
Publication TypeJournal Article
Year of Publication2010
AuthorsFischer LM, da Costa KAnn, Galanko JA, Sha W, Stephenson B, Vick J, Zeisel SH
JournalAm J Clin Nutr
Volume92
Issue2
Pagination336-46
Date Published2010 Aug
ISSN1938-3207
KeywordsAdult, Betaine, Choline, Diet, Dietary Supplements, Diet Records, Female, Genotype, Humans, Lactation, Maternal Nutritional Physiological Phenomena, Methylenetetrahydrofolate Reductase (NADPH2), Milk, Human, Nutrition Policy, Phosphatidylcholines, Phosphatidylethanolamine N-Methyltransferase, Phosphorylcholine, Polymorphism, Single Nucleotide, Pregnancy, Young Adult
Abstract

<p><b>BACKGROUND: </b>Choline is essential for infant nutrition, and breast milk is a rich source of this nutrient. Common single nucleotide polymorphisms (SNPs) change dietary requirements for choline intake.</p><p><b>OBJECTIVE: </b>The aim of this study was to determine whether total choline intake and/or SNPs influence concentrations of choline and its metabolites in human breast milk and plasma.</p><p><b>DESIGN: </b>We gave a total of 103 pregnant women supplemental choline or a placebo from 18 wk gestation to 45 d postpartum and genotyped the women for 370 common SNPs. At 45 d postpartum, we measured choline metabolite concentrations in breast milk and plasma and assessed the dietary intake of choline by using a 3-d food record.</p><p><b>RESULTS: </b>On average, lactating women in our study ate two-thirds of the recommended intake for choline (Adequate Intake = 550 mg choline/d). Dietary choline intake (no supplement) correlated with breast-milk phosphatidylcholine and plasma choline concentrations. A supplement further increased breast-milk choline, betaine, and phosphocholine concentrations and increased plasma choline and betaine concentrations. We identified 5 SNPs in MTHFR that altered the slope of the intake-metabolite concentration relations, and we identified 2 SNPs in PEMT that shifted these curves upward. Individuals who shared sets of common SNPs were outliers in plots of intake-metabolite concentration curves; we suggest that these SNPs should be further investigated to determine how they alter choline metabolism.</p><p><b>CONCLUSION: </b>Total intake of choline and genotype can influence the concentrations of choline and its metabolites in the breast milk and blood of lactating women and thereby affect the amount of choline available to the developing infant. This study was registered at clinicaltrials.gov as NCT00678925.</p>

DOI10.3945/ajcn.2010.29459
Alternate JournalAm. J. Clin. Nutr.
PubMed ID20534746
PubMed Central IDPMC2904035
Grant ListP30 ES010126 / ES / NIEHS NIH HHS / United States
AG09525 / AG / NIA NIH HHS / United States
DK55865 / DK / NIDDK NIH HHS / United States
DK034987 / DK / NIDDK NIH HHS / United States
DK56350 / DK / NIDDK NIH HHS / United States
RR00046 / RR / NCRR NIH HHS / United States