Dose-response to 3 months of quercetin-containing supplements on metabolite and quercetin conjugate profile in adults.

TitleDose-response to 3 months of quercetin-containing supplements on metabolite and quercetin conjugate profile in adults.
Publication TypeJournal Article
Year of Publication2013
AuthorsCialdella-Kam L, Nieman DC, Sha W, Meaney MPat, Knab AM, R Shanely A
JournalBr J Nutr
Volume109
Issue11
Pagination1923-33
Date Published2013 Jun
ISSN1475-2662
KeywordsAdult, Aged, Aged, 80 and over, Antioxidants, Ascorbic Acid, Dietary Supplements, Dose-Response Relationship, Drug, Drug Interactions, Female, Humans, Male, Middle Aged, Niacin, Quercetin
Abstract

<p>Quercetin, a flavonol in fruits and vegetables, has been demonstrated to have antioxidant, anti-inflammatory and immunomodulating influences. The purpose of the present study was to determine if quercetin, vitamin C and niacin supplements (Q-500 = 500 mg/d of quercetin, 125 mg/d of vitamin C and 5 mg/d of niacin; Q-1000 = 1000 mg/d of quercetin, 250 mg/d of vitamin C and 10 mg/d of niacin) would alter small-molecule metabolite profiles and serum quercetin conjugate levels in adults. Healthy adults (fifty-eight women and forty-two men; aged 40-83 years) were assigned using a randomised double-blinded placebo-controlled trial to one of three supplement groups (Q-1000, Q-500 or placebo). Overnight fasted blood samples were collected at 0, 1 and 3 months. Quercetin conjugate concentrations were measured using ultra-performance liquid chromatography (UPLC)-MS/MS, and metabolite profiles were measured using two MS platforms (UPLC-quadrupole time-of-flight MS (TOFMS) and GC-TOFMS). Statistical procedures included partial least square discriminant analysis (PLS-DA) and linear mixed model analysis with repeated measures. After accounting for age, sex and BMI, quercetin supplementation was associated with significant shifts in 163 metabolites/quercetin conjugates (false discovery rate, P<0·05). The top five metabolite shifts were an increase in serum guaiacol, 2-oxo-4-methylthiobutanoic acid, allocystathionine and two bile acids. Inflammatory and oxidative stress metabolites were not affected. PLS-DA revealed a clear separation only between the 1000 mg/d and placebo groups (Q(2)Y = 0·763). The quercetin conjugate, isorhamnetin-3-glucuronide, had the highest concentration at 3 months followed by quercetin-3-glucuronide, quercetin-3-sulphate and quercetin diglucuronide. In human subjects, long-term quercetin supplementation exerts disparate and wide-ranging metabolic effects and changes in quercetin conjugate concentrations. Metabolic shifts were apparent at the 1000 mg/d dose; further research is required to understand the health implications of these shifts.</p>

DOI10.1017/S0007114512003972
Alternate JournalBr. J. Nutr.
PubMed ID23151341