The genome-wide early temporal response of Saccharomyces cerevisiae to oxidative stress induced by cumene hydroperoxide.

TitleThe genome-wide early temporal response of Saccharomyces cerevisiae to oxidative stress induced by cumene hydroperoxide.
Publication TypeJournal Article
Year of Publication2013
AuthorsSha W, Martins AM, Laubenbacher R, Mendes P, Shulaev V
JournalPLoS One
Volume8
Issue9
Paginatione74939
Date Published2013
ISSN1932-6203
KeywordsBenzene Derivatives, Biomarkers, Gene Expression Profiling, Gene Expression Regulation, Fungal, Genome, Fungal, Oligonucleotide Array Sequence Analysis, Oxidants, Oxidative Stress, RNA, Fungal, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins
Abstract

<p>Oxidative stress is a well-known biological process that occurs in all respiring cells and is involved in pathophysiological processes such as aging and apoptosis. Oxidative stress agents include peroxides such as hydrogen peroxide, cumene hydroperoxide, and linoleic acid hydroperoxide, the thiol oxidant diamide, and menadione, a generator of superoxide, amongst others. The present study analyzed the early temporal genome-wide transcriptional response of Saccharomyces cerevisiae to oxidative stress induced by the aromatic peroxide cumene hydroperoxide. The accurate dataset obtained, supported by the use of temporal controls, biological replicates and well controlled growth conditions, provided a detailed picture of the early dynamics of the process. We identified a set of genes previously not implicated in the oxidative stress response, including several transcriptional regulators showing a fast transient response, suggesting a coordinated process in the transcriptional reprogramming. We discuss the role of the glutathione, thioredoxin and reactive oxygen species-removing systems, the proteasome and the pentose phosphate pathway. A data-driven clustering of the expression patterns identified one specific cluster that mostly consisted of genes known to be regulated by the Yap1p and Skn7p transcription factors, emphasizing their mediator role in the transcriptional response to oxidants. Comparison of our results with data reported for hydrogen peroxide identified 664 genes that specifically respond to cumene hydroperoxide, suggesting distinct transcriptional responses to these two peroxides. Genes up-regulated only by cumene hydroperoxide are mainly related to the cell membrane and cell wall, and proteolysis process, while those down-regulated only by this aromatic peroxide are involved in mitochondrial function.</p>

DOI10.1371/journal.pone.0074939
Alternate JournalPLoS ONE
PubMed ID24073228
PubMed Central IDPMC3779239
Grant ListR01 GM068947 / GM / NIGMS NIH HHS / United States
R01 GM080219 / GM / NIGMS NIH HHS / United States
GM068947 / GM / NIGMS NIH HHS / United States