Unexpected improvement in stability and utility of cytochrome c by solution in biocompatible ionic liquids.

TitleUnexpected improvement in stability and utility of cytochrome c by solution in biocompatible ionic liquids.
Publication TypeJournal Article
Year of Publication2006
AuthorsFujita K, Forsyth M, MacFarlane DR, Reid RW, Elliott GD
JournalBiotechnol Bioeng
Volume94
Issue6
Pagination1209-13
Date Published2006 Aug 20
ISSN0006-3592
KeywordsAnimals, Biocompatible Materials, Cytochromes c, Enzyme Activation, Enzyme Stability, Horses, Ions, Myocardium, Protein Conformation, Solutions
Abstract

<p>Proteins generally are only stable in vitro for short periods of time. This results in challenges during isolation and purification of recombinant proteins and reduces the shelf life of protein-based pharmaceuticals. Here we show that certain novel, biocompatible ionic liquids provide a stabilizing solvent for proteins, for example, cytochrome c, such that structure and activity are maintained even after 6 months of storage at room temperature. Normally, this protein would be rendered inactive after only 1 week in buffered aqueous solution. The effect of the ionic liquid solvent appears to be related to protection against hydrolysis.</p>

DOI10.1002/bit.20928
Alternate JournalBiotechnol. Bioeng.
PubMed ID16615145