VSL#3 probiotic modifies mucosal microbial composition but does not reduce colitis-associated colorectal cancer.

TitleVSL#3 probiotic modifies mucosal microbial composition but does not reduce colitis-associated colorectal cancer.
Publication TypeJournal Article
Year of Publication2013
AuthorsArthur JC, Gharaibeh RZ, Uronis JM, Perez-Chanona E, Sha W, Tomkovich S, Mühlbauer M, Fodor AA, Jobin C
JournalSci Rep
Volume3
Pagination2868
Date Published2013 Oct 08
ISSN2045-2322
KeywordsAnimals, Cell Transformation, Neoplastic, Colitis, Colorectal Neoplasms, Disease Models, Animal, Intestinal Mucosa, Mice, Mice, Knockout, Microbiota, Probiotics
Abstract

<p>Although probiotics have shown success in preventing the development of experimental colitis-associated colorectal cancer (CRC), beneficial effects of interventional treatment are relatively unknown. Here we show that interventional treatment with VSL#3 probiotic alters the luminal and mucosally-adherent microbiota, but does not protect against inflammation or tumorigenesis in the azoxymethane (AOM)/Il10⁻/⁻ mouse model of colitis-associated CRC. VSL#3 (10⁹ CFU/animal/day) significantly enhanced tumor penetrance, multiplicity, histologic dysplasia scores, and adenocarcinoma invasion relative to VSL#3-untreated mice. Illumina 16S sequencing demonstrated that VSL#3 significantly decreased (16-fold) the abundance of a bacterial taxon assigned to genus Clostridium in the mucosally-adherent microbiota. Mediation analysis by linear models suggested that this taxon was a contributing factor to increased tumorigenesis in VSL#3-fed mice. We conclude that VSL#3 interventional therapy can alter microbial community composition and enhance tumorigenesis in the AOM/Il10⁻/⁻ model.</p>

DOI10.1038/srep02868
Alternate JournalSci Rep
PubMed ID24100376
PubMed Central IDPMC3792409
Grant ListR01 DK073338 / DK / NIDDK NIH HHS / United States
R01 DK047700 / DK / NIDDK NIH HHS / United States
P30 DK034987 / DK / NIDDK NIH HHS / United States
R01 DK47700 / DK / NIDDK NIH HHS / United States
P40 RR18603 / RR / NCRR NIH HHS / United States
T32 DK007737 / DK / NIDDK NIH HHS / United States
R01 DK73338 / DK / NIDDK NIH HHS / United States