Identification and Characterization of a Class of MALAT1-like Genomic Loci.

TitleIdentification and Characterization of a Class of MALAT1-like Genomic Loci.
Publication TypeJournal Article
Year of Publication2017
AuthorsZhang B, Mao YS, Diermeier SD, Novikova IV, Nawrocki EP, Jones TA, Lazar Z, Tung C-S, Luo W, Eddy SR, Sanbonmatsu KY, Spector DL
JournalCell Rep
Date Published2017 05 23
KeywordsAnimals, Base Sequence, Cell Nucleus, Genetic Loci, Genome, Human, Humans, Lizards, Male, Nucleic Acid Conformation, Organ Specificity, RNA, Long Noncoding, RNA, Small Interfering, Spermatocytes

<p>The MALAT1 (Metastasis-Associated Lung Adenocarcinoma Transcript 1) gene encodes a noncoding RNA that is processed into a long nuclear retained transcript (MALAT1) and a small cytoplasmic tRNA-like transcript (mascRNA). Using an RNA sequence- and structure-based covariance model, we identified more than 130 genomic loci in vertebrate genomes containing the MALAT1 3' end triple-helix structure and its immediate downstream tRNA-like structure, including 44 in the green lizard Anolis carolinensis. Structural and computational analyses revealed a co-occurrence of components of the 3' end module. MALAT1-like genes in Anolis carolinensis are highly expressed in adult testis, thus we named them testis-abundant long noncoding RNAs (tancRNAs). MALAT1-like loci also produce multiple small RNA species, including PIWI-interacting RNAs (piRNAs), from the antisense strand. The 3' ends of tancRNAs serve as potential targets for the PIWI-piRNA complex. Thus, we have identified an evolutionarily conserved class of long noncoding RNAs (lncRNAs) with similar structural constraints, post-transcriptional processing, and subcellular localization and a distinct function in spermatocytes.</p>

Alternate JournalCell Rep
PubMed ID28538188
PubMed Central IDPMC5505346
Grant ListP01 CA013106 / CA / NCI NIH HHS / United States
P30 CA045508 / CA / NCI NIH HHS / United States
R01 GM042694 / GM / NIGMS NIH HHS / United States